Abdul Basit is a Principal Scientist 3 at Charles River's Worcester Discovery Bioanalysis group in Massachusetts, where he leads large molecule bioanalysis. In this role he oversees characterization and quantification of oligonucleotides, proteins, and endogenous metabolites using cutting edge technologies in omics science.
Dr. Basit has been in research and development for over 12 years, working at various research organizations in a myriad of roles. Prior to joining Charles River in 2021, he worked as Assistant Professor at Washington State University, and Senior Fellow/Technical Director for quantitative proteomics and metabolomics at the University of Washington. He also has working experience with in vitro DMPK and bioanalysis at Sai Life Sciences and Vanta Bioscience, India.
Dr. Basit received his Bachelor of Pharmacy degree from Pune University, India and Master’s degree in pharmaceutical science from the National Institute of Pharmaceutical Education and Research, India. He conducted his PhD in Drug Discovery from Istituto Italiano Di Technologia (Università degli Studi di Genova), Italy. Dr. Basit has authored and co-authored more than 35 peer-reviewed articles, over 30 conference abstracts, one book chapter, and is the co-inventor of two US patents.
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Publications
- Parvez MM, Basit A, Jariwala PB, Gáborik Z, Kis E, Heyward S, Redinbo MR, Prasad B. Quantitative Investigation of Irinotecan Metabolism, Transport, and Gut Microbiome Activation. Drug Metab Dispos. 2021 Aug;49(8):683-693. doi: 10.1124/dmd.121.000476. Epub 2021 Jun 1. PubMed PMID: 34074730.
- Ahire DS, Basit A, Karasu M, Prasad B. Ultrasensitive Quantification of Drug-metabolizing Enzymes and Transporters in Small Sample Volume by Microflow LC-MS/MS. J Pharm Sci. 2021 Jul;110(7):2833-2840. doi: 10.1016/j.xphs.2021.03.020. Epub 2021 Mar 28. PubMed PMID: 33785352.
- Balhara A, Basit A#, Argikar UA, Dumouchel JL, Singh S, Prasad B. Comparative Proteomics Analysis of the Postmitochondrial Supernatant Fraction of Human Lens-Free Whole Eye and Liver. Drug Metab Dispos. 2021 Jul;49(7):592-600. doi: 10.1124/dmd.120.000297. Epub 2021 May 5. PubMed PMID: 33952609. #Equal contribution first author.
- Chapa R, Li CY, Basit A, Thakur A, Ladumor MK, Sharma S, Singh S, Selen A, Prasad B. Contribution of Uptake and Efflux Transporters to Oral Pharmacokinetics of Furosemide. ACS Omega. 2020 Dec 29;5(51):32939-32950. doi: 10.1021/acsomega.0c03930. eCollection 2020 Dec 29. PubMed PMID: 33403255; PubMed Central PMCID: PMC7774078.
- 5. Basit A, Neradugomma NK, Wolford C, Fan PW, Murray B, Takahashi RH, Khojasteh SC, Smith BJ, Heyward S, Totah RA, Kelly EJ, Prasad B. Characterization of Differential Tissue Abundance of Major Non-CYP Enzymes in Human. Mol Pharm. 2020 Nov 2;17(11):4114-4124. doi: 10.1021/acs.molpharmaceut.0c00559. Epub 2020 Oct 2. PubMed PMID: 32955894.
- Visigalli D, Capodivento G, Basit A, Fernández R, Hamid Z, Pencová B, Gemelli C, Marubbi D, Pastorino C, Luoma AM, Riekel C, Kirschner DA, Schenone A, Fernández JA, Armirotti A, Nobbio L. Exploiting Sphingo- and Glycerophospholipid Impairment to Select Effective Drugs and Biomarkers for CMT1A. Front Neurol. 2020;11:903. doi: 10.3389/fneur.2020.00903. eCollection 2020. PubMed PMID: 32982928; PubMed Central PMCID: PMC7477391.
- Basit A, Prasad B, Estergreen JK, Sabath DE, Alade N, Veenstra DL, Rettie AE, Thummel KE. A Novel LC-MS/MS Assay for Quantification of Des-carboxy Prothrombin and Characterization of Warfarin-Induced Changes. Clin Transl Sci. 2020 Jul;13(4):718-726. doi: 10.1111/cts.12757. Epub 2020 Mar 9. PubMed PMID: 32004415; PubMed Central PMCID: PMC7359935.
- Zhang H, Wolford C, Basit A, Li AP, Fan PW, Murray BP, Takahashi RH, Khojasteh SC, Smith BJ, Thummel KE, Prasad B. Regional Proteomic Quantification of Clinically Relevant Non-Cytochrome P450 Enzymes along the Human Small Intestine. Drug Metab Dispos. 2020 Jul;48(7):528-536. doi: 10.1124/dmd.120.090738. Epub 2020 Apr 29. PubMed PMID: 32350063.
- Zhang H, Basit A, Wolford C, Chen KF, Gaedigk A, Lin YS, Leeder JS, Prasad B. Normalized Testosterone Glucuronide as a Potential Urinary Biomarker for Highly Variable UGT2B17 in Children 7-18 Years. Clin Pharmacol Ther. 2020 May;107(5):1149-1158. doi: 10.1002/cpt.1764. Epub 2020 Feb 12. PubMed PMID: 31900930; PubMed Central PMCID: PMC7167343.
- Basit A, Radi Z, Vaidya VS, Karasu M, Prasad B. Kidney Cortical Transporter Expression across Species Using Quantitative Proteomics. Drug Metab Dispos. 2019 Aug;47(8):802-808. doi: 10.1124/dmd.119.086579. Epub 2019 May 23. PubMed PMID: 31123036.
- Li CY, Hosey-Cojocari C, Basit A, Unadkat JD, Leeder JS, Prasad B. Optimized Renal Transporter Quantification by Using Aquaporin 1 and Aquaporin 2 as Anatomical Markers: Application in Characterizing the Ontogeny of Renal Transporters and Its Correlation with Hepatic Transporters in Paired Human Samples. AAPS J. 2019 Jul 11;21(5):88. doi: 10.1208/s12248-019-0359-1. PubMed PMID: 31297641; PubMed Central PMCID: PMC7413827.
- Li CY, Basit A, Gupta A, Gáborik Z, Kis E, Prasad B. Major glucuronide metabolites of testosterone are primarily transported by MRP2 and MRP3 in human liver, intestine and kidney. J Steroid Biochem Mol Biol. 2019 Jul;191:105350. doi: 10.1016/j.jsbmb.2019.03.027. Epub 2019 Apr 5. PubMed PMID: 30959153; PubMed Central PMCID: PMC7075494.
- Hamid Z, Basit A#, Pontis S, Piras F, Assogna F, Bossù P, Pontieri FE, Stefani A, Spalletta G, Franceschi P, Reggiani A, Armirotti A. Gender specific decrease of a set of circulating N-acylphosphatidyl ethanolamines (NAPEs) in the plasma of Parkinson's disease patients. Metabolomics. 2019 May 3;15(5):74. doi: 10.1007/s11306-019-1536-z. PubMed PMID: 31053995; PubMed Central PMCID: PMC6499742. #Equal contribution first author.
- De Vita T, Albani C, Realini N, Migliore M, Basit A, Ottonello G, Cavalli A. Inhibition of Serine Palmitoyltransferase by a Small Organic Molecule Promotes Neuronal Survival after Astrocyte Amyloid Beta 1-42 Injury. ACS Chem Neurosci. 2019 Mar 20;10(3):1627-1635. doi: 10.1021/acschemneuro.8b00556. Epub 2018 Dec 11. PubMed PMID: 30481470.
- Vozella V, Basit A#, Piras F, Realini N, Armirotti A, Bossù P, Assogna F, Sensi SL, Spalletta G, Piomelli D. Elevated plasma ceramide levels in post-menopausal women: a cross-sectional study. Aging (Albany NY). 2019 Jan 8;11(1):73-88. doi: 10.18632/aging.101719. PubMed PMID: 30620722; PubMed Central PMCID: PMC6339790. #Equal contribution first author.
- Bhatt DK, Mehrotra A, Gaedigk A, Chapa R, Basit A, Zhang H, Choudhari P, Boberg M, Pearce RE, Gaedigk R, Broeckel U, Leeder JS, Prasad B. Age- and Genotype-Dependent Variability in the Protein Abundance and Activity of Six Major Uridine Diphosphate-Glucuronosyltransferases in Human Liver. Clin Pharmacol Ther. 2019 Jan;105(1):131-141. doi: 10.1002/cpt.1109. Epub 2018 Jul 12. PubMed PMID: 29737521; PubMed Central PMCID: PMC6222000.
- Zhang H, Basit A, Busch D, Yabut K, Bhatt DK, Drozdzik M, Ostrowski M, Li A, Collins C, Oswald S, Prasad B. Quantitative characterization of UDP-glucuronosyltransferase 2B17 in human liver and intestine and its role in testosterone first-pass metabolism. Biochem Pharmacol. 2018 Oct;156:32-42. doi: 10.1016/j.bcp.2018.08.003. Epub 2018 Aug 4. PubMed PMID: 30086285; PubMed Central PMCID: PMC6188809.
- Basit A, Amory JK, Prasad B. Effect of Dose and 5α-Reductase Inhibition on the Circulating Testosterone Metabolite Profile of Men Administered Oral Testosterone. Clin Transl Sci. 2018 Sep;11(5):513-522. doi: 10.1111/cts.12569. Epub 2018 Jun 19. PubMed PMID: 29877607; PubMed Central PMCID: PMC6132360.
- Bhatt DK, Basit A, Zhang H, Gaedigk A, Lee SB, Claw KG, Mehrotra A, Chaudhry AS, Pearce RE, Gaedigk R, Broeckel U, Thornton TA, Nickerson DA, Schuetz EG, Amory JK, Leeder JS, Prasad B. Hepatic Abundance and Activity of Androgen- and Drug-Metabolizing Enzyme UGT2B17 Are Associated with Genotype, Age, and Sex. Drug Metab Dispos. 2018 Jun;46(6):888-896. doi: 10.1124/dmd.118.080952. Epub 2018 Mar 30. PubMed PMID: 29602798; PubMed Central PMCID: PMC5938891.
- Di Pardo A, Amico E, Basit A, Armirotti A, Joshi P, Neely MD, Vuono R, Castaldo S, Digilio AF, Scalabrì F, Pepe G, Elifani F, Madonna M, Jeong SK, Park BM, D'Esposito M, Bowman AB, Barker RA, Maglione V. Author Correction: Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington's disease. Sci Rep. 2018 May 24;8(1):8266. doi: 10.1038/s41598-018-23083-1. PubMed PMID: 29795393; PubMed Central PMCID: PMC5967301.
- Merrill CB, Basit A#, Armirotti A, Jia Y, Gall CM, Lynch G, Piomelli D. Author Correction: Patch clamp-assisted single neuron lipidomics. Sci Rep. 2018 Apr 25;8(1):6772. doi: 10.1038/s41598-018-24605-7. PubMed PMID: 29691427; PubMed Central PMCID: PMC5915401. #Equal contribution first author.
- Di Pardo A, Basit A, Armirotti A, Amico E, Castaldo S, Pepe G, Marracino F, Buttari F, Digilio AF, Maglione V. De novo Synthesis of Sphingolipids Is Defective in Experimental Models of Huntington's Disease. Front Neurosci. 2017;11:698. doi: 10.3389/fnins.2017.00698. eCollection 2017. PubMed PMID: 29311779; PubMed Central PMCID: PMC5742211.
- Vozella V, Basit A, Misto A, Piomelli D. Age-dependent changes in nervonic acid-containing sphingolipids in mouse hippocampus. Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Dec;1862(12):1502-1511. doi: 10.1016/j.bbalip.2017.08.008. Epub 2017 Aug 30. PubMed PMID: 28855145.
- Capodivento G, Visigalli D, Garnero M, Fancellu R, Ferrara MD, Basit A, Hamid Z, Pastore VP, Garibaldi S, Armirotti A, Mancardi G, Serrati C, Capello E, Schenone A, Nobbio L. Sphingomyelin as a myelin biomarker in CSF of acquired demyelinating neuropathies. Sci Rep. 2017 Aug 10;7(1):7831. doi: 10.1038/s41598-017-08314-1. PubMed PMID: 28798317; PubMed Central PMCID: PMC5552737.
- Di Pardo A, Amico E, Basit A, Armirotti A, Joshi P, Neely MD, Vuono R, Castaldo S, Digilio AF, Scalabrì F, Pepe G, Elifani F, Madonna M, Jeong SK, Park BM, D'Esposito M, Bowman AB, Barker RA, Maglione V. Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington's disease. Sci Rep. 2017 Jul 13;7(1):5280. doi: 10.1038/s41598-017-05709-y. PubMed PMID: 28706199; PubMed Central PMCID: PMC5509685.
- Tran TN, Athanassiou A, Basit A, Bayer IS. Starch-based bio-elastomers functionalized with red beetroot natural antioxidant. Food Chem. 2017 Feb 1;216:324-33. doi: 10.1016/j.foodchem.2016.08.055. Epub 2016 Aug 20. PubMed PMID: 27596427.
- Basit A, Pontis S, Piomelli D, Armirotti A. Ion mobility mass spectrometry enhances low-abundance species detection in untargeted lipidomics. Metabolomics. 2016;12:50. doi: 10.1007/s11306-016-0971-3. Epub 2016 Feb 8. PubMed PMID: 26900387; PubMed Central PMCID: PMC4744830.
- Realini N, Palese F, Pizzirani D, Pontis S, Basit A, Bach A, Ganesan A, Piomelli D. Acid Ceramidase in Melanoma: EXPRESSION, LOCALIZATION, AND EFFECTS OF PHARMACOLOGICAL INHIBITION. J Biol Chem. 2016 Jan 29;291(5):2422-34. doi: 10.1074/jbc.M115.666909. Epub 2015 Nov 9. PubMed PMID: 26553872; PubMed Central PMCID: PMC4732224.
- Basit A, Piomelli D, Armirotti A. Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS. Anal Bioanal Chem. 2015 Jul;407(17):5189-98. doi: 10.1007/s00216-015-8585-6. Epub 2015 Mar 8. PubMed PMID: 25749796; PubMed Central PMCID: PMC4471391.
- Dotsey EY, Jung KM, Basit A, Wei D, Daglian J, Vacondio F, Armirotti A, Mor M, Piomelli D. Peroxide-Dependent MGL Sulfenylation Regulates 2-AG-Mediated Endocannabinoid Signaling in Brain Neurons. Chem Biol. 2015 May 21;22(5):619-28. doi: 10.1016/j.chembiol.2015.04.013. PubMed PMID: 26000748; PubMed Central PMCID: PMC4459529.
- Astarita G, Avanesian A, Grimaldi B, Realini N, Justinova Z, Panlilio LV, Basit A, Goldberg SR, Piomelli D. Methamphetamine accelerates cellular senescence through stimulation of de novo ceramide biosynthesis. PLoS One. 2015;10(2):e0116961. doi: 10.1371/journal.pone.0116961. eCollection 2015. PubMed PMID: 25671639; PubMed Central PMCID: PMC4324822.
- Armirotti A, Basit A, Realini N, Caltagirone C, Bossù P, Spalletta G, Piomelli D. Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: application to human subjects with neurodegenerative dementia. Anal Biochem. 2014 Jun 15;455:48-54. doi: 10.1016/j.ab.2014.03.019. Epub 2014 Apr 4. PubMed PMID: 24708938.
- Basu S, Basit A, Ravindran S, Patel VB, Vangala S, Patel H. Split calibration curve: an approach to avoid repeat analysis of the samples exceeding ULOQ. Bioanalysis. 2012 Oct;4(19):2375-89. doi: 10.4155/bio.12.219. PubMed PMID: 23088464.
Patents
- IP: 48585.01US1 – “A novel LC-MS/MS method for PIVKAII quantification: Characterization of warfarin induced changes in prothrombin des-carboxylation”
Inventors: Bhagwat Prasad, Kenneth E. Thummel, Abdul Basit, Allan E. Rettie - IP: 47911.02US1 – METHODS FOR TREATING TESTOSTERONE DEFICIENCY IN MEN AND METHODS FOR PRECISE DOSING OF UGT2B17 SUBSTRATE DRUGS”
Inventors: Bhagwat Prasad, Abdul Basit, Haeyoung Zhang, John Amory
Book Chapter
- Haeyoung Zhang, Abdul Basit, Bhagwat Prasad. Quantifying drug metabolizing enzymes and transporters by LC-MS/MS proteomics. Elsevier - Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters. 2020, 359-385