JAX® NSG® Mouse Variant Portfolio

NSG® Mice

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NSG Mouse Model Variants

NSG mouse models are highly immunodeficient mice designed for cancer xenograft modelling, stem cell biology, humanised studies, and infectious disease research.

Charles River is the exclusive distributor of JAX® Mice in Europe and breeder of JAX® NSG Mice and JAX® NSG-MHC DKO I/II Mice at its European facilities. Additional NSG mouse model variants may be imported via Charles River Europe for European clients.

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Note: Use of NSG Mice by commercial or for-profit entities requires a no-fee JAX® Leap License prior to shipping. This includes mice shipped as part of Charles River's Animal Model Evaluation Program. Please see terms of use.

Strain Name / CodeModel BenefitsStrain ConsiderationsResearch AreasReference
NSG® / (614)
NOD scid gamma

Bred in Charles River France, Germany, Italy, and UK
  • Engrafts the widest range of solid and hematological cancers, including ALL and AML

  • Most sensitive host for cancer stem cells when compared to NOD SCID or nude mice

  • Longer lifespan than NOD SCID; supports long-term engraftment studies and capabilities; >89 weeks median
  • No thymic lymphomas, can be used for long & short-term experiments


  • Sensitive to irradiation
  • N/A
Ishikawa et al., 2005

Shultz et al., 2005
NSG®-MHC I/II DKO / (718)
NOD.Cg-Prkdcscid H2-K1b-tm1Bpe H2-Ab1g7-em1Mvw H2-D1b-tm1Bpe Il2rgtm1Wjl/SzJ

Bred in Charles River Germany and UK
  • Most resistant to xeno-GVHD of all NSG variants



  • Extended human IgG half-life compared to B2m knockouts
  • Resistance to xeno-GVHD associated with reduced CD45+ cell expansion in vivo

  • Sensitive to irradiation
  • Transplant research
Brehm et al., 2019
NSG®-SGM3 / (013062)
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ

Available as import via Charles River
  • Increased CD4+ FoxP3+ regulatory T cell population after CD34+ humanization

  • Enhances human myelopoiesis and terminal differentiation after CD34+ humanization

  • Increased efficiency of engrafting human acute myeloid leukemia (AML)

  • Supports higher levels of human myeloid cell engraftment
  • Compromised human stem cell regeneration



  • Suppression of human erythropoiesis



  • Reduction of human B-lymphopoiesis
  • Immunology




  • Oncology
Nicolini et al., 2004 Wunderlich et al., 2010

Billerbeck et al., 2011 Miller, et al., 2013
NRG / (007799)
NOD Rag gamma

Available as import via Charles River
  • Long-term multilineage hematopoeitic stem cell repopulation similar to NSG mice

  • Engrafts human PBMC without irradiation similar to NSG

  • Engrafts a wide range of solid and hematological cancers

  • Greater tolerance to genotoxic agents compared to NSG
  • No thymic lymphomas; can be used for long & short-term experiments


  • Requires higher dose of irradiation to obtain human HSC engraftment

  • The serum half-life of human IgG is greatly reduced in NSG-B2m due to defective FcRn function
  • Immunology
Pearson et al., 2008 Brehm et al., 2010

Maykel et al., 2014
NSG®-A2 / (009617)
NOD.Cg-Mcph1Tg(HLA-A2.1)1Enge Prkdcscid Il2rgtm1Wjl/SzJ

Available as import via Charles River
  • High engraftment of HLA-A2-restricted immune cells after CD34+ humanization

  • Antibody responses improved in a Dengue virus infection model

  • Allow HLA restriction of developing human CD8 cells in humanized models, and provide improved platforms for viral infection and vaccine models
  • No thymic lymphomas - can be used for long-term experiments


  • Sensitive to irradiation
  • HIV and infectious disease


  • Immunology
Shultz et al., 2010

Strowig et al., 2009
NSG®-HLA-A2/HHD / (014570)
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A/H2-D/B2M)1Dvs/SzJ

Available as import via Charles River
  • Enable functional CD4+ T cell responses to viral infection after CD34+ humanization

  • Permit HLA restriction and enhance immune responses of human CD8 T cells

  • Antibody responses improved in a Dengue virus infection model
  • No thymic lymphomas - can be used for long-term experiments


  • Sensitive to irradiation
  • HIV and infectious disease
Shultz et al., 2010

Strowig et al., 2009
NSG®-DR1 / (012479)
NOD.Cg-Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dmz Prkdcscid Il2rgtm1Wjl/GckRolyJ

Available as import via Charles River
  • Useful for transplantation studies in the absence of xeno-GVHD
  • Reduced CD45+ cell engraftment compared to NSG
  • HIV and infectious disease

  • Immunology
Covassin et al., 2011
NSG®-Abo DR4 / (017637)
NOD.Cg-Prkdcscid Il2rgtm1Wjl H2-Ab1b-tm1Doi Tg(HLA-DRB1)31Dmz/SzJ

Available as import via Charles River
  • Useful for transplantation studies in the absence of xeno-GVHD

  • Develop allo-GVHD post-engraftment of DR4-negative CD4+ T cells

  • Show enhanced antibody responses, and human CD4+ T cells interact more effectively with human antigen presenting cells
  • Reduced CD45+ cell engraftment compared to NSG

  • Higher proportion of mice with no CD45+ cell engraftment as compared to NSG
  • HIV and infectious disease

  • Immunology
Covassin et al., 2011 Danner R et al., 2011
NSG® B2m / (010636)
NOD.Cg-B2mtm1Unc Prkdcscid Il2rgtm1Wjl/SzJ

Available as import via Charles River
  • Resistant to xeno-GVHD



  • Useful for studying mechanisms for xeno-GVHD
  • N/A
  • HIV and infectious disease

  • Transplant research
Covassin et al., 2013 King et al., 2008
NSG®-(KbDb)null / (023848)
NOD.Cg-Prkdcscid H2-K1b-tm1Bpe H2-D1b-tm1Bpe Il2rgtm1Wjl/SzJ

Available as import via Charles River
  • Attenuated xeno-GVHD development post-Hu-PBMC transplantation

  • High Hu-CD45+ cell engraftment

  • Useful for studying mechanisms for xeno-GVHD
  • Reduced survival post Hu-CD34+ cell transplantation compared to NSG mice
  • HIV and infectious disease

  • Transplant research
Covassin et al., 2013
NSG®-Tg(Hu-IL15) / (030890)
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(IL15)1Sz/SzJ

Available as import via Charles River
  • Increased abundance and function of human NK cells following CD34-humanization
  • Sensitive to irradiation
  • Immunology
Brehm et al., 2019
NBSGW / (026622)
NOD.Cg-KitW-41J Tyr+ Prkdcscid Il2rgtm1Wjl/ThomJ

Available as import via Charles River
  • Human CD34 cells engraft effectively without preconditioning irradiation

  • Longer lifespan than other immunodeficient Kit mutants

  • Increased human erythroid cells (bone marrow)
  • Although irradiation is not required for stem cell engraftment, NBSGW mice likely share the same sensitivity to genotoxic agents as other NSG strains
  • Immunology
McIntosh et al., 2015
NSG®-PiZ / (028842)
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(SERPINA1*E342K)#Slcw/SzJ

Available as import via Charles River
  • Engrafts human and allogeneic mouse hepatocytes without rejection
  • Shares the same sensitivity to genotoxic agents as other NSG strains
  • Transplant research
Borel et al., 2017
NSG®-TLR4 KO / (033704)
NOD.Cg-Tlr4lps-del Prkdcscid Il2rgtm1Wjl/SzJ

Available as import via Charles River
  • Residual mouse innate immune system cannot respond to Tlr4 agonists

  • After humanization, NSG-Tlr4 KO are ideal for studying human TLR4-specific responses against tumors
  • Shares the same sensitivity to genotoxic agents as other NSG strains
  • Transplant research
Aryee et al., 2019