Fast, Robust Antibody Engineering Services With Tumbler®

What can Tumbler do for you?

  • Provide simultaneous, multi-parameter antibody engineering and optimization for antibody candidates discovered from our antibody libraries, or from your own development.
  • Enhance the therapeutic or diagnostic performance of any antibody candidate, regardless of format.
  • Deliver fully human therapeutic-ready antibodies improved for affinity, potency, cross-reactivity, specificity, and biophysical attributes.
  • Retrofit antibodies to bind to variants of the original target, providing an avenue to repurpose drug and lead candidates for a cost-effective drug discovery pathway.

How does Tumbler work?

  • The Tumbler antibody optimization platform generates billions of variants from one or more starting clones.
  • We construct a custom-made library, then introduce in-house library diversity and liability-free CDR variants, grafted into a 100% human framework.
  • We utilize a CDR shuffling approach to minimize clonal redundancy and amino acid liabilities, while maximizing functional diversity and developability.
  • We employ unique panning strategies to enrich clones that accomplish antibody optimization goals.
  • Your optimized clones are delivered in as little as three months.

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What can antibody engineering services do for your antibodies?

Antibody Humanization Services

Tumbler can humanize antibody leads originating from non-human sources. Your antibody is "tumbled" through our proprietary technology, yielding antibody variants with human structure and behavior. This library-based antibody humanization service outperforms chimeric CDR grafting approaches. It can also be combined with affinity maturation within a single step.

Affinity Maturation

Tumbler provides an optimal affinity maturation tool that can increase your antibody candidate’s binding up to 130-fold. Tumbler campaigns yield an entire panel of antibodies with improved characteristics, from which you can select your optimal hits.

3D rendering of antibody molecules, to represent webinar on Tumbler® antibody optimization platform for antibody humanization and affinity maturation.

Highly Customizable Antibody Optimization Suite
Watch our on-demand webinar to learn how the Tumbler platform simultaneously achieves multiple antibody engineering goals.
Watch the webinar

 

Antibody Liability Removal

Ensuring your candidate is ready for the clinic is one of the most challenging steps in antibody discovery. Tumbler provides customizable optimization of your antibody candidate, allowing you to remove unwanted attributes and select for desired characteristics critical to therapeutic success. Characteristics to consider include thermostability, expression, immunogenicity, and IP coverage.

Target and Paratope Engineering

The Tumbler platform's unique engineering and build steps allow us to explore near-to-far sequence space while designing the library to meet your project goals.

  • Engineer cross-reactivity to antigen homologs from cynomolgus monkey, mouse, or other species.
  • Engineer true biepitopic antibodies – antibodies that bind to two different targets with the same paratope.

Sequence-Activity Relationships

The Tumbler antibody engineering service can provide valuable insights into sequence-activity relationships, including quantified amino acid selection pressure and positional weight matrices. This information can guide future development and engineering decisions of candidate clones.

digital rendering of a monoclonal antibody

Zero to Antibody Hero: Laying the Groundwork for Preclinical Success
Are you doing everything you can to de-risk your antibody candidates? What does it take to go to preclinical development with a high degree of confidence? View this webinar to follow the journey of an antibody ‘candidate hero’ as it progresses through the early discovery gauntlet.
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What to expect in a Tumbler antibody engineering campaign

In addition to a lead molecule optimized to your specific needs, a Tumbler campaign will yield the following deliverables:

  • Risk assessment, sequence, and structural characterization of your starting antibody.
  • Primary screening profile and sequences for up to eight plates of antibody variants (usually including kinetics)
  • Sequence activity relationship (SAR) report
  • Antibody humanization report (if applicable)
  • Flexible options to express hits as IgG or any desired format, with additional characterization as needed.

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Other Antibody Engineering Services

In addition to our Tumbler multi-parameter antibody optimization service, Charles River antibody engineering capabilities also include antibody reformatting. Antibody leads discovered with our scFv phage display libraries, for example, can be reformatted into IgG antibodies, with several Fc backbones available (e.g., human IgG1, human IgG4, mouse IgG1, and mouse IgG2a). We handle the cloning, expression, and purification across several batch sizes.

We also have experience reformatting antibodies for more complex downstream applications, including CAR-T, ADCs, nanobodies, and bispecific antibodies. The Tumbler platform, along with our phage display antibody libraries, are just the starting points for our unique end-to-end antibody discovery and development services. Our integrated target-to-clinic portfolio also includes:

Contact an antibody specialist

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Frequently Asked Questions (FAQs) About Tumbler Antibody Engineering Services

  • Can any antibody be optimized?

    Yes, the Tumbler antibody humanization service is very flexible. In addition, we perform a free evaluation of your sequence before any agreement or contract is signed.

  • Does the Tumbler antibody humanization service work with any target?

    Yes, we have successfully optimized antibodies for:

    • Small molecules
    • Peptides
    • GPCR
    • Ion channels
    • Difficult viral targets
    • CAR-T constructs
  • How long does an antibody humanization campaign take?

    We begin by rapidly performing a free feasibility assessment, which outlines project approaches and possible risks. If needed, we will carry out some initial framework grafting, expression, and testing, which takes 2-4 weeks. The full bespoke library build and assessment takes 3-4 weeks. The generated libraries are then put through our Discovery workflow, which typically takes 8-12 weeks. Throughout the antibody humanization services workflow, there are go/no-go decision points at which the team and the client can decide to continue or not, offering additional setpoints to save time and money.

    We treat every project individually. Our experts work with your team to develop a custom antibody optimization work plan to obtain the antibody you need. We’re happy to have an evaluation call and provide specific timelines and deliverables for your antibody optimization project.

  • How many improved variants of my clone will I receive from a Tumbler antibody optimization campaign?

    Since you own the starting antibody, the full data package and all of the clone variants will be transferred to you as part of the project deliverables. Our experts will work with you to select the best possible candidates out of the panel of leads.