Traditional sequencing methods, such as Sanger and MALDI-TOF can be limited in what they can offer especially in situations where samples are not pure or cannot be cultured, when they fail to generate usable data, when the target DNA is large or where additional information at the genome level is required.
Next-Generation Sequencing (NGS) technologies shatter these limitations by addressing needs that cannot be met with compendial methods. When studying mixed environments, NGS allows for an understanding of populations without the need for sub-culturing. When it comes to pure isolates and/or deep sequencing of mixed environments, NGS allows for an understanding of organism genes and variants at a level never before possible.
In this on-demand, Tara Cassidy, NGS Account Manager of Microbial Solutions at Charles River, will provide you with a deeper understanding of how to leverage NGS to identify, classify, and understand microbes in mixed and pure samples by either targeting genes of interest or analyzing the entire genome.
Through the examination of real-world case studies, you will learn about:
- NGS technologies and bioinformatics: Unveiling the science behind NGS
- Regulatory insights: A comprehensive understanding of NGS regulations
Presenter:
Tara Cassidy B.Sc (AS), B.Sc (Hons), MASM
NGS Account Manager
Tara is a trained Microbiologist with expertise in animal science and Next-Generation Sequencing (NGS). With a background in microbiology and parasitology, Tara has experience with a wide range of technologies including DNA sequence-based assays, MALDI-TOF mass spectrometry, immunoassays, and NGS. She has been actively involved in the launch of Next-Generation Sequencing from research and development studies to a commercial product offering.