This case study on Sanger sequencing troubleshooting is a critical resource for professionals in pharmaceutical manufacturing and quality control. Learn how TAS can significantly improve the accuracy of microbial identification, overcoming the limitations of traditional Sanger sequencing, providing real-world applications of targeted amplicon sequencing, demonstrating its effectiveness in various scenarios where Sanger sequencing fails.
Solutions explored:
- Next-Generation Sequencing (NGS): Implementing NGS, specifically Targeted Amplicon Sequencing (TAS), provides a viable alternative, adept at handling mixed data, complex genetic variations, and non-isolated species
- TAS workflow and efficacy: The TAS workflow, involving DNA extraction, amplification, and bioinformatics, effectively processes samples that fail Sanger sequencing. This method has shown success in handling 81 samples, including both bacterial and fungal, that were previously unidentifiable
- TAS as a reliable backup: TAS serves as a dependable plan B when Sanger sequencing encounters technical limitations or difficulties in obtaining pure subcultures. Its ability to generate comprehensive microbial profiles within a single test revolutionizes microbial identification