Achieving Faster Results When Growth-Based Methods Are Slow or Ineffective
Our Technology and Market Development team has polled our QC pharmaceutical customers, and the top requests were the following:
- Expeditious and accurate results when investigating contamination events, so the customer may reduce downtime and uncertainty
- Deeper technical partnerships, with proven professionals who can offer support in contamination identification and response strategy, so effective meaningful action may be taken
- Single vendor management so the customer may manage their POs, experience dedicated customer service, and reduce administrative “busy-work” to focus on delivering results
In reaction to this feedback, our Accugenix® team built a service that covered all of these requests which resulted in:
- A more thorough resolution of organism genes and variants at a level never before seen
- Simultaneously sequencing multiple species from a sample and providing a snapshot of the microbiome
- Partnering with a global network of labs, bioinformaticians, and specialists who interpret your results, and provide post-report support
Accugenix NGS Case Study – Available for a limited time
Read this case study to learn how a pharmaceutical company used NGS to identify sources of contamination, track how organisms spread, and eliminate the sources of contamination.
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Accugenix® Next Generation Sequencing (Accugenix® NGS)
Accugenix® Next Generation Sequencing for bacterial identification and fungal ID simultaneously sequences millions of individual DNA fragments from a sample and provides a snapshot of the microbiome at that point in time. When investigating mixed species environments, it provides an understanding of microbial populations without the need for any culturing. Additionally, when studying pure isolates and/or sequencing of mixed species environments, NGS provides a deeper resolution of organism genes and variants at a level never before possible.
Investigations needing strain resolution may be opened due to an action level exceedance, a media fill or sterility test failure, or identification of an adverse trend in the EM tracking and trending data. While we maintain an extensive list of species with validated SLST/MLST schemes, if a validated strain typing scheme is not available, a new method may be developed using Sanger sequencing technology. If that development is not possible or information is needed as soon as possible, the Accugenix NGS strain comparison method can be used. This method was developed to compare protein-coding gene sequences inferred from whole genome sequencing data to generate a Strain Typing comparison report, offering the same high-resolution with a greater success rate of comparing target genes.
In addition to strain differentiation, the technology can be used to determine the sequence of entire genomes or targeted regions of DNA or RNA. These data have many applications such as:
- Identifying multiple species in a single sample, reducing the time and cost required for analysis
- When studying mixed species environments, you can understand microbial populations without the need for subculturing
- When studying pure isolates, you can understand organisms’ genes and variants to a degree that is not possible with traditional Sanger sequencing
- Providing identification in cases where Sanger sequencing was unsuccessful
Schedule a step-by-step walkthrough of our NGS services today.
Common Concerns About Next Generation Sequencing for Bacterial Identification and Fungal ID
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Are there validated methods for Accugenix NGS?
At this time there are very few GMP/validated NGS methods commercially available. When an ID is necessary, Accugenix NGS is better than no ID at all, especially if the original isolate was submitted for sequencing was due to its critical nature.
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Is Accugenix NGS complicated?
We understand that the process of generating and analyzing NGS data is challenging, often requiring specialized expertise and software. This can make adoption of in-house NGS daunting, which is why Accugenix has a team of specialists prepared to support you throughout the manufacturing processes.
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Is the vast amount of data generated by Accugenix NGS difficult to manage?
As with all our Accugenix® services, our team of trained experts interpret your reports for you and support you in designing a strategy to respond accordingly. In addition, Accugenix provides custom reports to fit the client’s needs, rather than providing an overwhelming bulk data report.
Bring Rare Bugs Around the World to Your Screen
Turn your device into a gallery of microbial science with 12 wallpapers featuring rare organisms identified across our global laboratory network.
Download Wallpapers
What are the applications for Accugenix NGS?
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Fungal and bacterial identification
- Pure or mixed species samples
- Non-culturable organisms
- Speciation in closely related taxa
- New species discovery
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Genomic analysis
- Microbiome analysis – Environmental monitoring and water system monitoring
- Microbial cell line characterization or authentication
- Toxin or virulence gene family detection
- Contamination detection
- Animal health monitoring
- Agricultural soil profiling
- Resistance gene detection
- Probiotic profiling
- Plasmid sequencing
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Subtyping
- Strain typing to support investigations
- Strain identification
Accugenix® NGS Fungal and Bacterial Identification Service Offerings
| Accugenix Whole Genome Sequencing (Accugenix WGS) | Analyzes the entire genome of a microorganism in a single test, reducing the time and cost required for analysis, while providing a high-resolution, base-by-base view of the genome |
| Accugenix Targeted Amplicon Sequencing (Accugenix TAS) | Identifies members of microbial populations in mixed species communities |
| Accugenix Plasmid Sequencing (Accugenix PLS) | Allows researchers to identify and characterize the plasmids in bacterial samples, providing insights into the bacterial traits and/or confirming the identity of plasmids used for bioprocess manufacturing |
Schedule a step-by-step walkthrough of our NGS services today.
Your Guide to Fast, Accurate Microbial Identification
With so many ID methods and providers out there, it’s tough to choose. We’ll guide you through how each test delivers results, so you can find the best fit for you.
Check it out