Endosafe® Nexus 200™:

The Nexus 200 automates every step, from dilution to result reporting, so you can run high-volume tests without constant monitoring. Once the deck is loaded and samples are scanned, the system locks the enclosure and executes a repeatable cycle:

• Barcode verification: Integrated 1D and 2D scanning confirms sample identity before processing, reducing mix-ups and transcription errors
• Automated sample handling: Four independent pipetting channels accelerate processing and maintain precision across up to 120 samples in one run
• Onboard cartridge management: A gravity-fed cartridge dispenser stages cartridges for the nexgen-MCS. A gripper then loads them into the system for rapid duplicate-channel BET testing and transfers used cartridges to an automated waste chute
• Unified data management (New in ESV 6.3): Central database integration aligns Nexus 200 with nexgen-PTS™, nexgen-MCS, and plate readers. Shared general settings, user management, permissions, audit trails, and data storage reduce setup time and simplify compliance
• Proactive run monitoring (New in ESV 6.3): Email alerts instantly notify if a system-level interruption occurs, helping teams respond faster and avoid delays
• Adaptive testing for R&D (New in ESV 6.3): Duplicate Dilution Mode preserves limited-volume samples and automates secondary dilution testing when needed, reducing waste and manual intervention

These enhancements work together to save time, reduce manual steps, and keep every run audit-ready.

Complex Dilution Capability for Diverse Applications
The Nexus 200 supports a wide range of testing needs, so you can run water, in-process, final product, and R&D samples in the same automated workflow. It performs serial dilutions up to 1:32,768 with multiple diluents available for use in the run. Complex or duplicate dilutions can be handled automatically, preserving sample material and avoiding manual intervention.

A Fully Enclosed, Safety-Focused Design
Operators and samples remain protected throughout every run:

• Doors lock automatically at run start and remain locked until the system stops
• Locking caster wheels provide stability and mobility for the integrated cabinet
• The enclosure prevents environmental interference that could affect results

Error Prevention and Real-Time Oversight
By limiting manual steps, the Nexus 200 reduces opportunities for variability and retests. Real-time alerts keep runs audit-ready and enable overnight operation with minimal supervision. The combination of barcode scanning, automated cartridge handling, and system-level monitoring ensures data integrity from first pipette movement to result.

Traditional BET keeps skilled analysts occupied with manual dilutions, curve preparation, and pipetting, work that carries a high risk of error but low value for advanced scientific expertise. The Nexus 200 minimizes these tasks, allowing analysts to focus on analysis, investigation, and proactive process control.

Standardized workflows across sites reduce training time, harmonize method settings, and lower the risk of deviations. Automation also shortens laboratory and out-of-specification investigations, improving efficiency and throughput without compromising compliance.

EndoScan-V powers Nexus 200’s data integrity and connectivity so you can manage results confidently across your lab network.

• Data security: Backup and restore features protect critical test data
• LIMS integration: Send results directly to your lab information management system
• Audit readiness: Searchable audit trails, permissions, and digital signatures maintain full traceability and authenticity
• Global Data Standards: Meets 21 CFR Part 11 and global GMP data integrity requirements

With the Cortex^™ add-on, you can monitor process control across instruments and sites in real time. ESV extends this with unified database management across Nexus 200, nexgen-PTS, and nexgen-MCS, shared user controls, and proactive email alerts for system-level events.

The Nexus 200 is validated to meet global compendial and regulatory expectations so you can operate with confidence across sites and regions.

• FDA-licensed kinetic chromogenic method (LAL cartridge) since 2006, fully compliant with USP, EP, and JP
• Supports sustainability with either 95% less LAL usage per test or the use of non-animal-derived Trillium recombinant cascade reagent (rCR) cartridges
• Designed for GMP QC labs in pharma, biotech, and medical device manufacturing

To keep systems running smoothly and in compliance, a dedicated Field Service Team provides preventive maintenance, repairs, and annual calibration. Validation services are available remotely, by sending samples to a Charles River lab, or on-site during extended training at installation. This ensures automation is fully operational and audit-ready from day one.

Yes, the Nexus 200 is powered by Endosafe EndoScan-V for LIMS integration and improved traceability, security, and data management. Charles River Cortex software, our endotoxin analysis software for investigative evaluation and process monitoring can be added to track and trend your endotoxin data in real-time.

Yes, the integrated cabinet is always included with the system and is not optional. The Nexus 200 system cannot be removed from the cabinet as it is designed to ensure stability of the system, integration of the waste chute, easy installation and the flexibility needed to maintain the system from all sides.

The robotic nature of the reagent handling, pipetting, and analysis employed by the Endosafe Nexus 200 means that human interaction is not necessary, therefore reduces variability in technical and transcription errors. Traditional endotoxin test methods require a laboratory technician to perform additional steps such as creating a standard curve, dilutions, rehydrating reagents, and even visual analysis of results. Additionally, automating your endotoxin testing allows you to remain complaint with data integrity standards by ensuring data are attributable, legible, contemporaneous, original, and accurate. Lab automation greatly reduces the chance of human-caused errors in the analysis, eliminates data integrity concerns over results interpretation, and maintains a high throughput environment in the laboratory by reducing additional work. 

Both 1D and 2D are readable so you can match your lab’s existing standards.

All components, in addition to preventive service, maintenance, and repairs of the Endosafe Nexus 200 automated endotoxin testing system are supported directly by Charles River.