Animal Model of Carrageenan-Induced Paw Edema
Charles River conducts contract studies in an established animal model of carrageenan-induced paw edema to test the efficacy of novel therapeutics. This model provides rapid assessment of a compound’s ability to provide anti-inflammatory and analgesic activity. Agents that show activity in this assay include NSAIDs, glucocorticoids, and coxibs (modest).
Animals are treated with compound prior to challenge with λ-carrageenan in one hind footpad. The contralateral footpad is injected with saline. Footpad thickness is measured at specified time points. Determination of tissue and/or serum cytokine levels and histologic analysis can also be performed.
Paw Edema Study Endpoints
- Clinical scores
- Paw volume measurements (plethysmometer)
- Tactile allodynia (Von Frey fibers)
- Thermal hypersensitivity (modified Hargreaves)
- Histopathology
- Cytokine analysis
- Clinical chemistry
- PK/PD blood analysis