Rapid beta-D-Glucan Detection

With recent inspections indicating a need to verify the absence of glucans, the assurance that your product is free of contamination is essential. Endosafe® beta-glucan cartridges provide a rapid, in-process test designed for investigational purposes to ensure that products do not contain (1,3)-β-D glucans. The test can quantify glucans faster, which supports tighter process monitoring and quicker out-of-specification (OOS) resolution.

Explore the Beta-Glucan Assay Guide

Two scientists holding up and looking at a single Endosafe Cartridge

Endosafe® Cartridge Technology
This brochure details endotoxin testing techniques and shows how cartridge technology can streamline workflows, shorten turnaround times, and improve precision compared to traditional methods.
Simplify your BET

  • Features
    • Employs the Limulus enzyme cascade to detect the presence of (1,3)-b-D glucan
    • Interfaces with our suite of scalable, automated Endosafe cartridge technology platforms
    • Designed to mimic kinetic chromogenic methods by measuring color intensity directly related to the (1,3)-b-D glucan concentration
    • Each cartridge contains precise amounts of glucan-specific LAL formulation, chromogenic substrate, and glucan standard
    • Results in approximately 30 minutes
    • Sensitivity range of 10-1,000 pg/mL
  • Applications
    • LAL endotoxin OOS results
    • Yeast protein production
    • Cell culture fluid
    • Air quality
    • Cellulose filter preparation
  • Test Procedure
    • Insert the cartridge into an Endosafe cartridge technology platform
    • Pipette 25 μL of a sample into each of the four sample reservoirs of the cartridge, then press "Start" to begin the test
    • The system draws and mixes the sample with the LAL reagent in two channels (the sample channels) and the LAL reagent and positive product control in the other two channels (the spike channels)
    • The sample is incubated and then combined with the chromogenic substrate
    • After mixing, the optical density of the wells is measured and analyzed against an internally archived standard curve
    • The results are then displayed within the software in less than 30 minutes
Reducing Horseshoe Crab Raw Material Usage

Is Your BET Program Sustainable?
We recognize every organization sets its own path toward the 3R principles while protecting product quality and patient safety. Our portfolio has changed how we approach endotoxin detection, with options that draw on animal-free reagents, a recycling pathway for used cartridges, and reduced reliance on horseshoe crab-derived material.
Go Animal-Free