Transgene Copy Number
Transgenes typically insert randomly in the genome as tandem repeats, but the site of insertion and number of copies varies in each founder line. Using qPCR, we determine relative copy number for each founder line, providing valuable information such as transgene instability and segregation of closely linked insertions, both commonly overlooked factors influencing the consistent phenotype of a colony. Undetected alterations may be passed through the germline, making copy number determination an important quality control tool for your transgenic models.
The relative or absolute transgene copy number may also help explain changes of phenotype or inconsistent performance of the colony, both crucial for studies such as disease phenotype and preclinical drug efficacy analysis.
These services are only available in Europe. Regardless of your location, if you would like to speak with a member of our team about gene copy number determination or any other topic, please fill out our contact form.
Tackling Research Reproducibility: Genetic Reporting Guidelines and Validation Methodologies
View this webinar to learn about the requirements of LAG-R guidelines and how to best adopt them in your research, including verifying strain authenticity, mutant alleles, and transgene copy numbers, as well as identifying genetic anomalies and reducing experimental risks. Watch on Demand