Zygosity Determination
It’s often difficult to discriminate between homozygous and hemizygous transgenic animals using traditional PCR, but the distinction between these zygosities is critical to transgenic breeding strategies, as well as planning study cohorts.
We'll provide a definitive report on the zygosity of your animals quickly and accurately using our high-throughput, state-of-the-art qPCR technology.
These services are only available in Europe. Regardless of your location, if you would like to speak with a member of our team about zygosity testing or any other topic, please fill out our contact form.
Technical Parameters
- Measurement of fold difference from known heterozygous control
- Two known heterozygous controls used for calculations
- Both target and endogenous control are tested in quadruplicate
- Analysis is performed using delta-delta CT – relative quantitation method
Tackling Research Reproducibility: Genetic Reporting Guidelines and Validation Methodologies
View this webinar to learn about the requirements of LAG-R guidelines and how to best adopt them in your research, including verifying strain authenticity, mutant alleles, and transgene copy numbers, as well as identifying genetic anomalies and reducing experimental risks. Watch on Demand