Orthogonal Screening Platforms to Confirm Hits

Charles River offers a range of orthogonal platforms for primary screening, fragment screening, hit confirmation and characterization, hit-to-lead, and lead optimization.

Surface Plasmon Resonance (SPR):
  • A fully equipped suite offering a range of Biacore platforms, complemented with Bruker instrumentation
  • Enabled for low, medium, and high throughput analyses
  • Binding confirmation, kinetic profiling, MOA investigations (including competition studies), and in-house fragment screening
Biochemical Assays:
  • Many readouts accommodating high-concentration fragment screening
  • Multi-modal fluorescence and luminescence readouts
  • Binding assays and many other formats
Cellular Assays:
X-Ray Crystallographic Screening:
  • Integrated Structural Biology and Protein Science department allows for crystallization using fresh protein
  • Weekly slots at both UK and European synchrotron facilities for high-resolution data acquisition
  • Access to XCHEM fragment screening at the Diamond Synchrotron, Didcot, UK
Nuclear Magnetic Resonance (NMR):
  • Access to 600 MHz, five-channel Bruker Avance III spectrometer with a 24-place sample changer and a cryoprobe which can observe a wide range of biologically relevant nuclei at high sensitivity
  • Fragment screening using in-house fluorinated fragment library
  • Ligand observed binding through STD, CPMG, and WaterLOGSY experiments
  • Protein observed ligand binding, backbone assignments, and mapping of ligand binding site
SAMDI-MS:
  • Combination of surface chemistry and MALDI TOF MS
  • Premier label-free and high-throughput assay
  • Analysis of biochemical (functional) activities and affinity selection MS to discover small molecule binders to protein and oligonucleotide (RNA targets)

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