PBMC Humanized Mouse Models for Preclinical Research
Peripheral blood mononuclear cells (PBMCs) play a crucial role in humanizing immunodeficient mice, offering a powerful platform to evaluate potential therapeutics for diseases such as cancer and infectious diseases. PBMC humanized mouse models are surrogates for human clinical trials, enabling human cellular immune function assessment. These adult immune cells engraft rapidly, making them ideal for short-term studies focused on T cell modulation.
Take the Next Step with Our Humanized Mice Portfolio
Not sure where to start? Contact us for a quote, to place an order, or to speak with our scientific experts about your research needs.
Create Your Own PBMC Humanized Mouse Models with Our Exclusive Kit
Timing is everything in research, but sometimes, you’re not quite ready to begin your study. Our exclusive PBMC Select Humanization Kit offers a convenient, reliable solution for generating PBMC-humanized mouse models exactly when needed.
Our kit gives you greater flexibility in study timing—a crucial advantage when working within a limited therapeutic window, such as slow tumor growth rates. By supplying the mice and cells together, the PBMC Select Kit ensures that humanization aligns seamlessly with your study timeline.
Additionally, our use of pre-qualified and recallable PBMC donors eliminates the need for independent qualification, saving you valuable research time and keeping costs under control.
These donor cells have been screened and pre-qualified for engraftment rate, body weight loss, and study duration. Furthermore, cell numbers are optimized specifically for the NCG mouse model, ensuring consistent and reliable engraftment without a license.
The PBMC Select Humanization Kit is compatible with three NCG Plus Portfolio models to generate these humanized options:
- HuPBMC NCG
A triple-immunodeficient mouse, lacking functional/mature T, B, and NK cells, with reduced macrophage and dendritic cell function. These animal models can host xenograft cells, tissue, and human immune system components. - HuPBMC NCG-B2m-KO
A triple-immunodeficient NCG mouse with a B2m (β2-microglobulin) gene knockout. B2m is a component of the MHC complex essential for MHC I expression. NCG-B2m-KO mice express low levels of MHC I, leading to a delayed onset of graft-versus-host disease (GvHD). - HuPBMC NCG-MHC-dKO
A triple-immunodeficient NCG model lacking expression of MHC class I molecule (H2-K and H2-D1) and MHC class II molecule (H2-Ab1). The decreased expression of MHC I and MHC II leads to a significant delay in the onset of GvHD in PBMC humanized mice.
How to Thaw Cryopreserved Cells (Vials) Technical Protocol
The technical protocol includes helpful tips to ensure the successful thawing of your cells. Use this protocol to follow along with the instructional video.
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What are the Benefits of Using the PBMC Select Humanization Kit?
| Flexibility and timeliness | Start your research faster and on schedule with your study timelines. |
| Cost-effective | Avoid unnecessary costs by preparing only the exact number of models needed for your study. |
| Reduced risk | Minimize variables by handling the humanization process in-house. |
| Quality | Our PBMC cell inventory has been screened for engraftment rate, body weight loss, and study term. Cell numbers have been optimized for use in the NCG mouse model. |
| From a trusted source | This kit is the product of a partnership among industry leaders with 100 years of combined experience in providing high quality research models and human biologics to the research industry. |
POSTER
Donor Selection Matters
See how PBMC donor selection affects engraftment rates and onset of graft-versus-host disease in humanized NCG mice in cancer studies.
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Frequently Asked Questions (FAQs) About PBMC Humanized Mouse Models and Kits
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What are PBMCs?
Peripheral blood mononuclear cells (PBMCs) are a type of white blood cells that are important to immunity including lymphocytes, monocytes, or macrophages.
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Can I create my own PBMC humanized mice?
Yes. You can use our NCG/PBMC Select Humanization Kit to create your own line of humanized mice. Orders for the NCG/PBMC Select Humanization Kit can be placed by contacting [email protected].
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What comes in a Charles River PBMC Select Humanization Kit?
Each kit includes an NCG, NCG-B2m-KO, or NCG-MHC-dKO model order and enough PBMCs to engraft them at the recommended dose.
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What are the benefits of using a PBMC Select Humanization Kit?
Our PBMC Select Humanization Kit offers a convenient, reliable solution for generating PBMC-humanized mouse models exactly when needed. By supplying the mice and cells together, the PBMC Select Kit ensures that humanization aligns seamlessly with your study timeline.
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Are the models in the PMBC Select Humanization Kit irradiated?
We do not typically use myeloablation before PBMC engraftment, as it can shorten the study window.
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How do I order an NCG/PBMC Select Humanization Kit?
Orders for the NCG/PBMC Select Humanization Kit can be placed by contacting [email protected].
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How do you thaw vials of PBMCs?
Our technical protocol includes helpful tips to ensure the successful thawing of your cells. Follow along with the instructional video using this protocol.