What is Recombinant Protein Production?
Charles River offers custom recombinant protein production services of high-quality recombinant proteins for all down-stream requirements. These may include, but are not limited to, biological assays or biophysical assays, in vivo studies, and structural solutions.
Our team of Protein Production Scientists are experts in engineering, focusing on challenging proteins that have little-to-no existing literature. The team designs constructs with a "right the first time" ethos, relying on their experience of successfully generating large quantities of crystallographic-grade proteins.
Breakthrough LINE-1 Reverse Transciptase Structure Published In Nature
Charles River protein scientists and crystallographers, as part of a global coordinated academic and industry effort led by ROME Therapeutics and Dr. Marty Taylor at Massachusetts General Hospital, have produced the first-ever high-resolution structure of LINE-1 reverse transcriptase (RT) using X-ray crystallography.
Read More
We then couple the most suitable recombinant protein expression host with multi-parameter studies to optimize the soluble protein yield, before progressing to large-scale production.
Utilizing automated protein purification service platforms, our aim is to purify your expressed protein as quickly as possible. Working as part of a multi-disciplinary Structural Biology team, we pride ourselves on our ability to deliver high quality crystallographic grade (>95 % purity) protein with the required pattern of post-translational modifications at the desired yield. We are experienced at working to low levels of endotoxin, a critical requirement for in vivo preparations.
We have a comprehensive suite of protein characterization techniques available in our lab, enabling monitoring throughout production (if required) or QC of fresh proteins post-production. We also offer protein characterization services for client-provided proteins.
Available Protein Expression and Purification Services:
- Construct design / protein engineering
- Multi-parallel construct generation
- Protein expression using E. coli, mammalian, insect cells-baculovirus, and BacMam
- Scalability from bench to fermenter scale (up to 20 L)
- Automated protein purification services complemented with an extensive range of chromatography techniques
- Crystallography grade protein quality (>95 % pure)
- Buffer Screening and Stability studies
- Labelled proteins for NMR
- Low endotoxin preparations for in vivo studies
- Characterization methods of SDS-PAGE, Western Blotting, analytical HPLC-SEC, DLS, DSF, MS, Tm
Backed by years of expertise, our Protein Expression and Purification Science specialists have successfully produced protein over an array of target classes and offer a comprehensive range of characterization techniques to complement our protein production services.
Frequently Asked Questions (FAQs) About Protein Production Services
-
Do we offer any other biophysical analysis?
We offer a full suite of complementary Biophysical techniques and can design your protein to ensure suitability for your project.
-
Can you provide protein for screening activities?
Our team is knowledgeable in the protein requirements of different down-stream research activities and can provide seamless integration with in-house screening activities, giving you support every step of the way.
-
There is no structure or literature about my protein of interest. Can you still provide protein expression services?
Recognized as experts in the design and expression of complex proteins, we can troubleshoot through the challenges of recombinant protein production to help you develop your de novo structural solution.